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In general, results of the plaque reduction assay fit in the middle range of IC50s of the other VI assays; better for some neutralizing reagents and viruses, worse for other neutralizing reagents and viruses (laboratory 9 in Figure 1 and Figure 2).
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As before, three subsequent assays must fall within specification to consider the assay fit-for-purpose for use in clinical trials (Lee et al, 2006).
Our finding that comp-1 sperm have reduced pseudopod lengths in an in vitro assay fits with their altered patterns of localization in vivo.
Overall, these data have shown that the selection window boundaries determined by agar plate assays fit well with results obtained in vivo in piglets for cefquinome.
In general, IC50s obtained in the plaque-reduction assays fitted in the middle of the spectrum of sensitivity of the other VI assays.
Since ToyJ does not saturate under the conditions of the assay, the fit provides a lower limit for this value.
The ALDEFLUOR assay may fit the universality required for a stem cell marker to be reliable across species and tissues.
Therefore, the FLICA assay is fit for the poly-caspases analysis, but it would be prudent to support this test with other specific methods for the detection of activated caspases, such as western blot analysis and measurement of caspases activity.
All urine specimens were diluted to achieve concentration for optimal density according to the ELISA kit instruction before performing an ELISA assay to fit the concentrations of respective NGAL protein in the linear range of the standard curve.
All urine specimens were diluted to achieve concentration for optimal density before performing the ELISA assay to fit the concentrations of respective NGAL protein in the linear range of the standard curve.
The TbUAP hits were repurchased, and 10-point inhibitor IC50 curves were determined using the discontinuous coupled colorimetric assay and fitting the dose response curve to a four-parameter fit in ActivityBase XE (IDBS).
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