Sentence examples for assay firstly from inspiring English sources

A novel type of cinnamic acid quinazoline amide derivatives (20 42), which designed the combination between quinazoline as the backbone and various substituted cinnamic acid as the side chain, have been synthesized and their biological activities were evaluated within cytotoxicity assay firstly and then potent EGFR inhibitory activity.

For ferric reducing antioxidant power (FRAP) assay, firstly, FRAP reagent was prepared by mixing TPTZ (2.5 mL, 10 mM in 40 mM HCl), 25 mL of 300 mM acetate buffer, and 2.5 mL of FeCl3·6H2O.

P-glycoprotein ATPase assays firstly demonstrated that evodiamine was a substrate of P-glycoprotein, while HP-β-CD and EVO/HP-β-CD inclusion complexes inhibited P-glycoprotein by blocking P-glycoprotein ATPase activity.

The content of cNOS in the supernatant was assayed firstly after HUVECs were incubated with calycosin in different concentration (0 μg/ml, 0.01 μg/ml, 0.1 μg/ml, 1 μg/ml, 10 μg/ml or 20 μg/ml, all for 24 h of incubation) or different time (all in 10 μg/ml, 0 h, 4 h, 8 h, 12 h, 24 h or 48 h) separately.

The performance of the genomagnetic assay was firstly demonstrated for a DNA synthetic target by its double-hybridization with both a digoxigenin probe and a biotinylated capture probe, and further binding to streptavidin-modified magnetic beads.

This assay was firstly established using HEK293 cells with sulforaphane as a positive control (Figure 5A).

To explore the functional role of NUF2 in pancreatic cancer cell growth, MTT assay was firstly performed in PANC-1 and Sw1990 cells.

For the HMF/GMF exposure assay, animals were firstly reared on the GMF for 7 days to adapt to the environment, and then randomly grouped as four mice per standard "shoebox" cage.

Assuming that benzyl acceptors could preserve the stabilizing stacking, benzyl β-d-glucoside firstly assayed as acceptor resulted in both poor yields and poor regioselectivity.

For this purpose, we firstly assayed whether paclitaxel treatment of PSPs and control clones (CCs) sublines induced alterations typically associated to aneuploidy appearance (chromosome missegregation and apoptosis defect).

To test whether let-7 is expressed in the correct cell type to act as a transmitter of steroid-modulated response to stress to control oogenesis in adults, we firstly assayed let-7 miRNA expression.