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TGFβ in supernatants was measured using the sensitive assay first described by Abe et al. [29] whereas IFNγ and IL-17 were measured using the Luminex platform.
Samples were analysed using a screening assay first.
We recognize that describing this assay first, as in the original manuscript, was confusing.
Mice were tested in the Y maze cognitive behavioral assay first.
To verify whether PLK1 mRNA is really targeted by mir3686, we conducted reporter assay first.
The Y2H assay first confirmed the interaction between zebrafish Amotl2a and zebrafish Yap1 and Taz (red in Figure 5B).
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HCV genotyping was carried out using Invader HCV genotyping assay (Third wave technology USA).
Clonogenic assays first expand tumour cells before treatment with cytotoxic agents.
10 μl of cell lysate was assayed first for firefly luciferase and then for Renilla luciferase activity.
The other compound, 'LRRK2-IN-1', is a leading LRRK2 kinase inhibition reagent in biochemical assays, first described in ref. (22).
The attenuative or restorative consequences of PMA or forskolin treatment on the interaction were established by GST pull-down assays first.
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