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The three files are called Investigation file, Study file, and Assay file.
The Study Assays section is used to record information about each assay and its assay file, particularly, the assay and technology types, measurement variable names, and the assay file names (see example in Figure 7B).
To capture the measurement data, ISA-TAB-Nano specifies new fields in the Investigation file and in the Assay file.
One may choose to enter the factor values either in the Study file and/or in the Assay file, as appropriate to the actual experimental procedure(s).
Animals were allowed to move freely for 1 hr before being immobilized by 1 μl of 1 M sodium azide (movie of bacterial choice assay, File S2).
Finally, the Assay file associates the raw data, derived data and image files via the column fields, Raw Data File, Derived Data File, and Image File, respectively.
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As a result, the Investigation, Study and Assay files are linked.
The Assay files are used to record the assay metadata along with references to the names (or URIs) of the raw/derived data and image files.
The ISA-TAB-Nano specification makes use of the ISA-TAB Investigandon, Study, Assayssay files to record and share the assay metadata.
The data in the Study and Assay files for each sample are integrated through the Sample Name fields found in both of the files.
Thus, the information of each biospecimen or material sample in the Study and Assay files can be linked to each other.
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