The genes we screened were ones that had been classified as having an Emb phenotype through any RNAi assay (feeding, soaking, or injecting) on WormBase.
The fact that these clones were not Emb in our experiments can be explained by the fact that, as previously noted, the genes screened were ones reported as having an Emb phenotype in WormBase through any RNAi assay (feeding, injecting, soaking, feeding in a sensitized strain background).
Because the feeding assay quantifies feeding in age-synchronized adult nematodes with fully developed nervous systems, it was necessary to separate the adults from their offspring for data analysis.
To determine if a gross difference in food intake could play a role in ndi1-mediated longevity, we assayed feeding behavior in esgGA L4>ndi1 flies and controls.
By using a blue-dye feeding assay combined with feeding observations (Wong et al., 2009), we found no significant effect of rapamycin on feeding behavior or on the amount of food consumed.
No genotoxic effect in E. coli K12 was observed in a host-mediated assay after feeding of 2,6-DMA to mice, nor was feeding or injecting it effective in a sex-linked recessive lethal assay in Drosophila melanogaster (58).
An independent assay of feeding using a modified dye-tracking assay [ 28] was used to parse the feeding behavior into the proportion of flies that feed within the assay period and the meal size of flies that feed.
The assay quantifies feeding in age-synchronized adult nematodes with fully developed nervous systems, and thus, it was necessary to separate the adults from their offspring.
