The most relevant of the standard factor assays for monitoring rFVIIa therapy is the FVII assay (factor VII clotting activity [FVII C]).
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Conditions were elucidated to enable pg/mL quantitation of the peptide in human plasma using steepest ascent to efficiently optimize assay factors.
The tool provides an intuitive web interface that allows users to import experimental data generated from a standardized experimental design, select the assay factors, run the standardized analysis algorithms, and generate tables, figures, and listings (TFL).
Thus, driving medicinal chemistry optimization in a cellular assay factors in cellular permeability and provides a physiologically relevant system for testing the inhibitor.
These differences may be related to cohort differences, such as the older mean age of the current study population, or assay factors.
The pooled data from all laboratories running the same assay were used to determine the smallest detectable interlaboratory fold difference and to test for the affects of assay factors on cytokine recovery.
When screening slow-growing mycobacteria such as M. tuberculosis, a 2 4-fold change in MICs can be rationalized due to differences in growth media, growth density, carbon source, amount of serum present, and other assay factors.
Since the spontaneous cytokine secretion impacts significantly on the sensitivity of this assay, factors that especially influence the non-specific spot production, possibly the medium type or serum source, will need to be systematically analyzed in a separate study.
Although results for different assays were significantly correlated (r = 0.38, p < 0.05, Spearman rank test), between-assay factors ranged from 2.0 to 217.9 (median 74.6) in individual patients.
As this conditioned media already contains SASP factors, we treated target cells with bafilomycin A to block protein secretion, and assayed factors within cell lysates after exposure to conditioned media from control or senescent VSMCs.
