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The mean (or median) differences observed between groups in these parameters were substantially greater than the variations due to assay errors.
The intraindividual variability, resulting from assay errors, model misspecifications, and other unexplained sources, was best described with a proportional error model.
Missingness of subjects of a particular genotype group from a population sample may be due to chance, genotyping assay errors, or clinical ascertainment bias related to that genotype.
Compared to the traditional plug assay, the sleek design of angioreactors provides a standardized platform for reproducible and quantifiable in vivo angiogenesis assays and prevents assay errors due to absorption of Matrigel by the mouse.
In our assay, errors that may arise because of physical variations between transwells and cell seeding irregularities are addressed by the use of RL-expressing cells that do not express miR-126 and a measure of cells that remain in the transwell (i.e., cells that did not invade).
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The discrete insulin and nutrition doses used and limits on allowed dosing changes from a prior intervention are defined in Table 3, where these limits provide robustness to assay error and patient safety.
When the XCI assay error varied between 0.03 and 0.05, the average number of clones in pauciclonal LCLs was estimated between 4 and 5.
However, based on our assessment that >3 CTCs may be the minimum number of CTCs required to sample heterogeneity and minimize assay error, this criteria would only be met in about 50% of patients in both our lung and breast cancer studies and this is an important limitation of current technologies.
From this evidence, dilutional or assay error accounts for <5% deviation between results.
Since no estimates of uniform assay error were available from the original studies, we utilized an assay error polynomial where standard deviation (SD) = 1.04 + (0.14 × C), where C is the drug concentration (in mg/liter).
Outer rows were not used and media were changed every 3 days to avoid assay error due to media evaporation.
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