The phrase "assay equation" is correct and usable in written English.
It can be used in scientific or technical contexts, particularly in chemistry or biology, to refer to a mathematical expression that describes the relationship between variables in an assay.
Example: "To determine the concentration of the substance, we applied the assay equation derived from our experimental data."
Alternatives: "analysis formula" or "testing equation".
The Pfaffl method is a particularly useful approach as it takes into account the PCR efficiency of each assay [ 48] (Equation 1).
PCR efficiency (E) was evaluated by performing a dilution series experiment using a target assay and the Equation E = 10(−1/slope)[ 65].
PCR efficiency (E) was evaluated by performing a dilution series experiment using a target assay and the equation E = 10(−1/sloPfafflfaffl 2001).
Both fits gave an apparent dissociation constant of 0.3 μM (Table 2), a value that overlaps the Kd obtained with the ELISA assay by using equation (1).
To determine the EP, the relative estrogenic potency (REP) of each steroid hormone, hormone conjugate, or phytoestrogen was first derived in the YES assay (Supporting Information equation SI-2), using starting concentrations ranging up to 100 mg/L.
Root mean square standardized effect [ 122] with sample size bias adjustment was calculated for each assay with the equation, and unbiased standardized pairwise mean differences (g) between wildtype PRE and each mutant within each assay were also calculated with the equation g = m j - m c MSerror × Γ 2 n − 2 2 Γ 2 n − 3 2 2 n − 2 2 [ 43].
Soluble transferrin receptor will be converted to be equivalent with the Flowers assay using the following equation: Flowers sTfR = 1.5 × Roche sTfR + 0.35 mg/L [ 58] then body iron will be calculated as the log ratio of sTfR to plasma ferritin concentration [ 58].
The average crystallite size was assayed with the Scherrer equation to be ~ 45 nm for the UC phosphors.
Here we provide the theoretical basis for the design of this assay derived from simple equations based on bimolecular interactions.
