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Microtissues produce therapeutic transgenes at increased levels and offer tissue-like assay environments to improve drug-function correlations in current discovery programs.
Such differences in the expression of response to selection in assay environments different from the maintenance environment have also been reported previously (Ackermann et al., 2001).
Shorter FFL in young chico 1 /+ flies does not appear to be caused by increased locomotor activity, as average activity counts were similar between chico 1 /+ and w 1118 controls (Fig. 2D), although, again, the assay environments were different.
A series of √2-fold dilutions of drug-containing medium (from 5120√2 to 80 mg L−1 for CFX, from 1280√2 to 20√2 mg L−1 for CHL, and from 80√2 to 5√2 mg L−1 for KM), together with a drug-free control, were used as the assay environments.
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And fourth, differences between the experimental assay environment and the respective selection regimens led to reduced courtship by ML-evolved males as an artefact.
When the assay environment matched the environment in which the female lineage had evolved, progeny viability was greater (interaction between selective environment and assay environment).
When modeled with ANOVA, the interaction between assay environment and selective environment was significant for Exp.
One possible reason might be the assay environment, which had equal sex ratio.
Microarray experiments were performed to assay environment-specific gene expression during exponential phase growth for each strain.
About 2 μL of each culture was inoculated into 198 μL of each assay environment and grown for 24 h.
A non-significant interaction suggests the difference in egg production between Evelyn Lake genotypes and all others did not depend on the assay environment.
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