Exact(11)
(c) A workflow illustrating the throughput of genes and shRNA constructs at different stages of the screen and the assay end points.
The assay end product was imaged with a NanoWizard-II BioAFM (JPK Instruments AG) operating in dynamic mode.
At the time of assay end point, MTT was added to the cultures and incubated for 3 4 h.
25 min was chosen as the assay end because most body parts were clean at that point.
Second, the drug activity database was generated using a single assay end point, that is, short-term growth inhibition and cytotoxicity.
Assay end points were measured fluorimetrically with a fluorescence spectrometer (FluoStar, BMG LabTech, Germany) and Optima program set at λexcitation=544 nm and λemission=590 nm.
Similar(49)
In order to confirm that the soluble cytosolic fluorescent signal of SOD1-GFP does not obscure inclusions, we treated the cells using the detergent saponin at the assay end-point, allowing the soluble SOD1-GFP protein to diffuse out of the cells while retaining the inclusions (Fig. 3C).
A CT value of 45 is the assay end-point and DNA considered 'undetectable'undetectable
A CT value of 45 is the assay end-point and when DNA is considered undetectable.
Serum albumin levels were measured using a colorimetric assay end-point method (Albumin Plus; Roche GmbH, Mannheim, Germany).
When one is assessing the utility of a laboratory test in monitoring rFVIIa therapy, it is important to consider factors such as availability, turnaround time, and relevance of assay end-points to clinical outcomes, among others [ 66].
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