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Generation of bromodomain mutant controls can establish assay efficacy even in advance of inhibitor development.
Cycle threshold (Ct) values were compared in a titration experiment to determine the assay efficacy.
However, similar to the aforementioned genotoxicity assays, issues with nanoparticle interference have been reported with traditional comet assay efficacy.
This latter analysis was undertaken in an attempt to assess whether trends could be strengthened if the assay variability was less and, on this basis, 22 patients were selected to have similar assay efficacy.
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By performing cytofluorimetric analyses and bromodeoxyuridine (BrdU) assay, the efficacy of nanocarriers to deliver the antineoplastic drug into a PEL cell line namely BCBL-1 (immortalized body cavity B-cell lymphoma) was investigated.
The 5-choice serial reaction time test (5-CSRTT), which originated from the continuous performance test (CPT) in humans, may serve as a useful translational assay for efficacy in these key behavioral domains.
Rubianol-d (43) and rubianol-e (44) exhibited the inhibition without cytotoxicity in the MTT assay whose efficacy was equivalent to L-NMMA's; rubianol-c (42), rubiarbonols A (16) and F (21), rubiarbonone C (27) showed both cytotoxic and inhibitory activities on NO production [19].
To assay compound efficacy, synchronized pha-1 Htn-Q150 pha-1 Htn-Q150 pha-1 Htn-Q150nd without drug for seven days, then scored for degeneration using the dye-fiL1ing assay (Figure 3A).
Furthermore, to validate the transwell migration assay, the efficacy of the CXCR2 antagonist SCH-527123 was tested.
The antibacterial activities were assessed in vitro by 1) Agar diffusion test (ADT) 2) MIC by microdilution method 3) Time kill assay 4) Efficacy in ex vivo dentine model 5) Haemolytic assay.
Although iodochlorohydroxyquinoline demonstrated activity in the ARE- bla-mut assay, the efficacy (21%) was considerably lower than in the ARE- bla assay (40%), supporting the activity of iodochlorohydroxyquinoline in the ARE pathway.
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