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Figure 4 Summary of the results of the viability/cytotoxicity assay, early apoptosis detection and ROS detection following incubation with increasing GC BTNPs concentrations.
In this assay, early apoptotic cells stained with Annexin V but not with PI while late apoptotic or necrotic cells stained with both Annexin V and PI.
Thus, the type of trait used to estimate resistance and the timing of a phenotypic assay (early or late in the infection process) could significantly influence the characterization of phenotypic and genetic variance.
For the tube formation assay, early (7-day culture) and late (14-day culture) endothelial progenitor cells were studied in the presence and absence of vascular endothelial growth factor (VEGF -A (3, 8).
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It may be necessary to assay earlier stages of trichome development using laser capture microdissection to find transcripts in early trichome formation in specific cell types.
For transformation assay, early-passage NIH3T3 cells were seeded at 1.5 × 10 cells per well in 6-well dishes the day before transfection with purified RasV12 or Src527F encoding plasmids (both at 0.5 μg).
Residual activity was determined after terminating the reaction with 0.3 mL of 10% (wv−1) TCA, as described in the standard protease assay earlier.
Furthermore, a [3H] or [125I] radiotracer can be used to establish binding assays early on to identify leads with potent binding affinity for PET consideration.
In vitro characterization (XIAP BIR3 and linker-BIR2 BIR3 linker-BIR2 BIR3 assays, early ADMET profiling) of the compounds has bindingrformed, identifying one lead for further in vitro and in vivo evaluation.
The prions in the conditioned media were assayed early on (P0) or after nine 1 20 splits (P9).
Acute haematological toxicity was assessed by daily blood counts, and an assessment of late marrow toxicity made by assaying early marrow progenitor cells using CFUgm assays.
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