It is possible that the tolerance defect we observed in the ethanol-reared adult flies might be due to a ceiling effect of the assay during the second ethanol exposure.
To assess the extent of WNV exposure to the flock, serum samples from 2 mixed-age cohorts were collected to test for specific antibodies to WNV and Saint Louis encephalitis virus (SLEV) by a constant virus serum dilution neutralization assay during the second site visit (7 ).
MO-injected embryos were assayed during the first three days of development using specific morphological and molecular criteria based on staging and anatomical landmarks as described previously[9].
To determine the effect of tellurite on ROS-responsive enzymatic behavior, the activities of catalase (CAT) and superoxide dismutase (SOD) were assayed during the first hour of tellurite treatment.
Decreasing the primer ratio to 5 1 balanced the accumulation of the allele-specific and alternate allele PCR products in allele-specific PCR assays during the second phase of amplification.
DOI: http://dx.doi.org/10.7554/eLife.01503.024 Consistently, by quantitative time-course ChIP assays performed during the first 48 hr of adipogenesis, we observed sequential enrichments of lineage-determining TF C/EBPβ, H3K4me1/2 methyltransferase MLL4, H3K4me1/2, and H3K27ac on C/EBPβ+MLL4+ adipogenic enhancers identified on Pparg gene locus.
The molecular beacon fluorescence in situ hybridization (FISH) assay used during the fourth infection trial used modified reverse PCR primer sequences for genogroup I and II viruses (28 ).
Serum for the cotinine assay was obtained during the first clinic visit but was only available for 981 of the 1,240 individuals with expression profiles.
The cytotoxic activity of ricin-treated cells evaluated against antigen-related target cells, in a short term incubation 51Cr release assay, was unaffected during the first 30 min after treatment but decreased with time over the next few hours.
For example using paraoxon as substrate, it was found that 10% of the samples assayed showed nonlinear kinetics during the first 15 min of the assay, linearity being established only after this time [ 78].
