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Supplemental Figure S6 disclosed very significant TAF single base pair assay discrimination, far in excess of that produced when 600 nM YOYO-1 and 40 mM TMA-Cl concentrations were used.
Genotyping was performed using multiplex SNaPshot technology with an ABI fluorescence-based assay discrimination method (Applied Biosystems, Foster City, CA, USA).
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By combining genus-specific and species-specific probes, the assay allowed discrimination of staphylococci, streptococci and enterococci as well as differentiation of therapy-relevant pathogens such as Staphylococcus aureus and Enterococcus faecium/durans.
All but two genetic variants were genotyped by commercially available TaqMan allelic discrimination assays (Assay-on-Demand and Assay-by-Design, Applied Biosystems, Foster City, CA).
Kinetic assays allow discrimination of competitive, non-competitive and un-competitive inhibitors.
Mutation screening was performed by two allele-specific PCR assays and discrimination of the allele-specific PCR fragments by agarose gel electrophoresis.
Accuracy was >99%, according to duplicate genotyping of 10% of all samples using the Taqman SNP genotyping assay-allelic discrimination method (Applied Biosystems).
We measured NPY, PENK and WIF1 by QM-MSP on two hundred and sixty six serum samples and assayed the discrimination power of their CMI.
Briefly, we used the Taqman® allelic discrimination assay (5' nuclease assay,[ 19]) performed on an ABI HT7900 (Applied Biosystems, Foster City, CA).
The APOE single nucleotide polymorphisms (SNPs) rs429358 and rs7412 were genotyped using Taqman SNP genotyping assays (determined by allelic discrimination assays based on fluorogenic 5′ nuclease activity) and the allele inferred.
The Assay-by-Design service (www.appliedbiosystem.com) was used to set up a Taqman allelic discrimination assay for the ACTN3 (rs1815739 C1747T).
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