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Cytocompatability of the scaffolds was assessed using MTT assay, direct contact test and cell attachment studies.
Cytocompatability of the composite scaffolds were assessed by MTT assay, direct contact test and cell attachment studies.
MTT assay, direct contact test, and cell attachment studies indicated that, the nano-composite scaffolds are better in scaffold properties and it provides a healthier environment for cell attachment and spreading.
Using a gene reporter assay, direct interaction of miR-20a with the LRF 3'UTR is demonstrated.
In contrast to the TBARS assay, direct HPLC analysis of phospholipid hydroperoxides did not give any indication of increased lipid peroxidation in Cftr−/− lung.
In this assay direct comparisons of myocyte shortening demonstrated that R193H myocytes are slower to relax than A63V mutants at 50 , 75 and 90% relaxation times (Table 1).
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This assay directed toward the dnaJ gene was tested on a total of 355 strains representing 13 Vibrio species and 17 non-Vibrio species.
The assay, directed towards the rpoA gene, was tested on a total of 287 samples representing six Vibrio species and ten non-Vibrio species.
INS-1 cell insulin secretion and insulin content were measured via enzyme-linked immunosorbent assay directed against rat insulin (ALPCO Diagnostics; Insulin (Rat) High Range EIA).
No PCR product was visible with either antibody in an assay directed at a non-specific site upstream of Jun (panel B) that had no match to this motif.
PCR assay directed toward the NS1 gene (3'ORF) was used for parvovirus detection [ 21].
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