Despite the apparent concordance between replicate runs, when we calculated viral gene copy number using calibration curves generated from individual qPCR runs, we observed inter-assay differences in the copy number values generated.
The discrepancy between the reported AR effects of the compounds might be caused by different sensitivities of the various assays, differences in co-factors in the different cell lines, and/or the use of different AR response elements (Sommer and Haendler 2003; Verrijdt et al. 2003).
While we saw some reduction in gp120 binding, it was difficult to quantitate the results due to the intra-assay differences in gp120 binding in the absence of antibody.
With this assay, gradual differences in the righting response can be observed (Hartenstein et al. 1996).
The paired-samples t-test was used to assay the differences in the miRNA expression levels between cancer tissues and corresponding adjacent tissues.
The PCR assay detects differences in the melting point temperatures for SPPV, GTPV and LSDV, obtained after fluorescence melting curve analysis.
Consequently, for each of these assays, apparent differences in the expression of a gene between two samples are actually differences in expression per unit of RNA or "per transcriptome" (Kanno et al. 2006).
For the inhibitor studies and the luciferase assays, difference in fold change over treatment was tested using a random-intercept mixed model.
An analysis of the coatings subjected to the saline mist assay revealed clear differences in the corrosion products formed, which were strongly adherent in the FS coating and only slight adherent in the EA coating, leading to loss in thickness.
We previously developed an in vivo PGC migration assay that identified differences in the signaling capacity of G protein gamma subunits.
However, the indirect method alone is accurate for measuring fly feeding during long-term, undisturbed, experimental conditions once assessed by the combined assay for any differences in the ingestion ratio.
