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Even though the primers used for this assay did not include CpG islands, a PCR bias (i.e. preferred amplification of unmethylated or methylated DNA), had to be excluded [ 30- 32].
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The hpVIR assay does not include HPV 68.
These assays did not include internal controls for DNA extraction and PCR amplification, however.
Additional comments have been added to the main text explaining that the bead motility assay does not included anionic lipids.
Some assays do not include an extraction step.
It is important to realize that the results from the competition assays do not include any information about the binding site or biochemical activity of the factor.
Furthermore, the flow chamber does not allow for pulsatile shear-flow conditions and realistic geometries similar to those encountered in small postcapillary venules, and most flow chamber assays do not include red blood cells.
Of note, the PCR array did not include an assay for miR-371.
Non-Mendelian transgene segregation has been noted in triticale [ 12], but since this observation was based on a histochemical reporter gene assay and did not include any DNA analysis, its basis could not be ascertained.
Cytotoxicity was estimated in all cell lines using the 2,3-bis 2-methoxy-4-nitro-5-sulfophenyl -5-[ phenylamino carbonyl]-2 H-tetrazolium hydroxide assay (2,3-bis 2-methoxy-4-nitro-5-sulfophenyl -5-[ phenylamino carbonyl]-2not include virus.
Another limitation was that we did not include an assay for testing anti-CCP3.
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