Binding of the conjugate at the test zone of the assay device is visible by the unaided eye and results may be read within 10 minutes.
An SAI value of 1 indicated a greater proportion of mosquitoes moved into the control chamber (away from the treatment end of the assay device) indicating a repellent action.
The composite strip is contained in a plastic assay device with a round sample well positioned above the sample application pad and a test result window positioned above the detection zone of the strip.
Tests are performed by the addition of 5 µl serum to the sample pad of the assay device followed by the addition of 130 µl sterile running fluid consisting of phosphate-buffered saline, pH 7.6, containing 1.67% bovine serum albumin and 3% Tween 20.
The platform used was an Illumina® Infinium II Multisample assay device.
In these experiments, assay device enclosures were cut from adhesive-coated Melinex sheets (Fralock, San Carlos, CA) using the CO2 laser cutter.
On day 9 after plating the cells, the BAM plates were transferred to the Myomics' Myoforce Assay Device (MAD).
Thus, lower cost and rapid assay device make this method interesting for early assessment of myocardial reperfusion.
One key characteristic of most of these newer paper-based devices that remains consistent with early LFTs is the inclusion of dried reagents in the assay device.
Briefly, 10 μl whole blood was applied to the sample well of the assay device immediately followed by the addition of 120 μl running fluid.
