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To shorten the diagnostic window period, the first point-of-care HIV assay, Determine HIV ½ Ag/Ab Combo (D4G, Alere, I) for the combined detection of p24 and anti-HIV antibody has been recently marketed and mainly tested in high prevalence setting.
HIV testing was done on-site using a rapid lateral flow assay (Determine® HIV-1/2, Alere, Massachusetts, USA) and, if positive, a confirmatory test was done using a different rapid lateral flow assay (Unigold™ Recombigen® HIV, Bray, Ireland).
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Finally, an ELIspot assay determined that the magnitude and kinetics of responses differed between previously infected and naı̈ve monkeys.
To develop a novel and automated assay determining plasma thiol/disulphide homeostasis, which consists of thiol disulphide exchanges.
The Lyme multiplex assay determines antibodies to three antigens, called 'outer surface proteins (Osp)', of B. burgdorferi which have been shown to correlate with vaccinal antibodies, or acute and chronic stages of Lyme disease.
The static loading assay determined the deformations, through strain gages (single-element gage, two-element gage and three-element rosettes), and the displacements along the sleeper, using linear displacement transducers.
The CLIA assay determined 61%9090) of the 148 cases to be positive and 39% (58) as negative.
(C) WST-8 assay determined the viability of HUVECs treated with the nanoparticles for 48 h at different concentrations (**P < 0.01, ***P < 0.001 vs normal, n = 3).
The detection limit of the assay, determined by spiking with 100, 10 and 1 TCID50 virus per flask, was approximately 1 TCID50/ml.
The assay determines the number of viable cells in culture based on quantification of the ATP present.
Reproducibility and repeatability of the assay, determined by blind testing kidney samples from 20 confirmed positive and 20 negative rodents both appeared 100%.
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