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The concentrations required for 50%% growth inhibition on the roots and shoots of the test plants in the assay (defined as I50) were determined by a logistic regression (Y = b + a log10X. Y; % length, X; concentration, a; slope, b; intercept) analysis (Table 1).
The analytical limit of detection of the ELISA assay, defined as the concentration corresponding to the mean signal+3 SD of 10 replicates of the zero calibrator was 5.4 µg/L.
For the purpose of this study, ER-positivity was defined as at least moderate nuclear ER staining in more than 10% of tumor cells, and HER2 positivity was documented by either 3+ immunohistochemical staining with anti-HER2 antibody in >30% of tumor cells, or by a positive fluorescent in-situ hybridization assay, defined as >2.2 HER2 gene copies per tumor cell.
cNormal C-reactive protein (CRP) by high-sensitivity assay, defined as ≤0.287 mg/dl.
The sensitivity of the assay, defined as the maximum tolerable dilution within the reproducibility range was 5%.
The cryoglobulin screening assay, defined here as "rapid screening test," is based on the same physical principle (light scattering detection), although some modifications have been introduced.
Similar(46)
Additionally, the screening assay defines newly formed macropinosomes as endocytic organelles >0.5 µm in diameter formed within a 5 minute window of dextran internalisation.
The spectrophotometric assay defines the Hb saturation level at a given PO2 and yields standard oxygen-binding curves.
Since the sulfation assay defines the arrival of cargo at a TPST-containing compartment, we examined the cellular site of the two human TPST1 and TPST2 isoforms [ 45, 46] in the presence and absence of Exo2.
For myelination assays, defined medium was supplemented with 1 mM dibutyryl cAMP for 48 h unless otherwise stated.
Standardized reference laboratory assays defined HER2 amplification in a large cohort of patients (n = 469) with pancreatic ductal adenocarcinoma (PDAC).
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