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Data obtained from the lactate dehydrogenase (LDH) release assay confirmed the neurotoxicity results of the MTT assay (data not shown).
However, neither OsSMF1 nor MBD1 was expressed by OsSMF1 in the protoplast assay (data not shown).
However, no interference was observed with the ROS production assay (data not shown).
Ten-100 μg/ml of recombinant proteins were initially examined for anti-fungal activity assay (data not shown).
AuNR exposure caused no significant effects on the cell viability, as measured by the MTT assay (data not shown).
These findings were reproduced for the lipL32 assay (data not shown).
Neither thioredoxin nor glutathione served as reductants for LiΔTXN3 in this assay (data not shown).
These results have been confirmed by a tritium-labeled AdoMet incorporation assay (data not shown).
Similar results were obtained using PNNAG proliferation assay (data not shown).
ThyX activity was also verified by NADPH oxidation assay (data not shown).
This treatment did not affect responsiveness in the LDH release assay (data not shown).
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