MA was responsible for the study conception and design, manuscript writing, establishment of the synovial explant assay, data collection and analysis and critical revision of the manuscript.
Paola Del Serrone carried out research concept and design, collection and/or assembly of biological assay data, collection and/or assembly of molecular biology assay data, data analysis and interpretation, statistical analysis, writing the paper, critical revision of the paper, and the final approval of the paper.
JGK, NGA, and JTS performed immunoblotting, biochemical assays, data collection, and data analysis.
ARL performed all molecular assays, data collection and analysis, and drafted the manuscript.
PL performed genetic and immunological assays, data collection and statistical analyses and participated in interpretation of data.
We developed assay meta data collection tools for the biological assays to focus on the factors most likely to influence cytotoxicity (e.g. cell-line, incubation time, dose, endpoint detection method).
ETB participated in the tissue homogenization and ELISAs, TBARS and Total Antioxidant assays, and data collection.
The technique enables novel types of assays and allow data collection at a level of resolution not previously obtained.
Baseline, 6- and 12-month serum levels of insulin, glucose, IGF1, IGF-binding protein 3 (IGFBP3), adiponectin, and leptin were assayed, and after data collection, homeostasis model assessment of IR (HOMA-IR) scores were calculated.
