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The invasion assay correlation, shown in Figure 7B, shows a different pattern.
Thus, the lack of ECL array-comet assay correlation for styrene does not negate the genotoxicity pathway and dynamics results found in our cell-free assays, which may still be relevant to humans.
Strikingly, although the compounds had broadly the same rank order of potency as in the Back Assay (correlation coefficient R = 0.78 following the removal of a single outlier), the IC50's were, on average, 30-fold lower and the Hill slopes median was 1.1 with 10 of 21 compounds tested giving Hill slopes ≤1 (Table 1 and Supplementary Table S3).
For three subjects, we compared cytokine measurements for TNF-α obtained by the multiplex assay with measurements obtained using an enzyme-linked immunosorbent assay (Quantikine, R&D Systems, Minneapolis, MN) to validate the multiplex assay (correlation between assay measurements: R = 0.966; data not shown).
The level of conservation, estimated by the phastCons score [ 70] and phyloP [ 71], had weakly positive associations with level of activity in the transient transfection assay (correlation coefficients from linear regression R = +0.173 and R = +0.015, respectively, Additional file 2: Figure S14B).
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Furthermore LINCO-plex assay, RnD Systems Fluorokine-MAP assay and the Bio-Rad 17-plex correlation of 13 cytokines showed a positive correlation between LINCO 29-plex and the RnD Systems Fluorokine-MAP assay for most of the cytokines, whereas the Bio-Rad 17-plex assay correlations to LINCO 29-plex assay and the RnD Systems Fluorokine-MAP assay were frequently negative.
Levels of in vitro-, in vivo-, and ex vivo-induced DNA damage were measured using the Comet assay; correlations between damage levels with in vitro cell survival and follow-up clinical data were investigated.
Inter- and intra-assay correlation of variance was 7.36% and 3.84% respectively.
Overall, the MassCode system exhibited good inter-assay correlation, which was examined over a 13 day period.
Figure S5 demonstrates the agreement between methylation values determined independently for these loci by HELP and MassArray (with a between-assay correlation in this case of R = −0.96353).
For both IFN-γ-secreting and IL-2-secreting T cells, responses to PPD and ESAT-6 and CFP-10 peptide pools were equally strongly correlated between the colour and fluorescent immunospot assays (Figure 2) indicating that inter-assay correlation is independent of the antigen used.
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