Exact(1)
Strikingly, in this simple assay correct proteasome-dependent Def1 processing was observed, with full-length Def1 processed to the shortened version.
Similar(59)
Counts were averaged in each assay, corrected for background counts and for BCR-ABL expression, as determined by Western blotting.
The final yield after purification was determined using the total protein results obtained by the BCA assay corrected for the rHA purity determined by SDS-PAGE.
The number of migrating cells in each experiment was corrected for proliferation effects using a cell viability assay (corrected migrating cell number=counted migrating cell number/percentage of viable cells).
The correction value was then added to each result from the different cytokine assays: corrected C T = C T target + HK correction value.
In this study, we developed a cobalt albumin binding (CAB) assay to correct the flaws of the ACB test with improving the sensitivity and precision.
A positive serum pool was included in each assay to correct interassay variations.
An appropriate internal control was maintained in each assay to correct for the spiked base line for absolute IL-6 quantification.
A virus titration was included to check the virus dose of the assay was correct and a titration of horse antisera positive for AHSV-4 VN antibodies were included in the assay as positive controls.
Equimolar inclusion of this primer (150 nM) in the ARMS-DCA assay yielded correct allele calls for the 12 samples under scrutiny and completely suppressed detectable non-specific 7028C product formation, without observable negative effects on the typing of pure and deliberately admixed 7028C/2706A and 7028T/2706G samples (26- and 32-cycle ARMS).
The SNP assay gave correct genotypes an average of 93%% of the time (Table II).
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