DD and CK designed and developed the assay, coordinated the study and drafted the manuscript.
Further studies with the mouse assay and the in vitro assay coordinated with physiochemical characterization of test soils can confirm and extend the results obtained in this study and identify refinements in experimental design and data analysis that can improve the accuracy and reliability of estimates of bioaccessibility and bioavailability.
A cDNA microarray chip assay revealed coordinated down regulation of genes encoding enzymes of the cuticular wax biosynthetic pathway in the glossy mutant, with BnCER1 being one of the most severely suppressed genes.
RPH conceived the study, supervised the qPCR assays, and coordinated work.
Pyrosequencing assay design, genomic coordinates, assay conditions and assay validation are described in detail in references 27, 71 and Murphy SK et al., submitted.
In order to identify the genes in the copy number altered regions it was necessary to translating BACs coordinate used in the comparative genomic hybridization (CGH) assays into genome coordinates.
Marine sticklebacks showed clear indications of schooling behavior in this assay: they exhibited polarized, coordinated movement with the model school (Video S1; Fig. 4) [4], [4].
HS performed the PCR assay and sequencing, and coordinated the study.
SRM-assays encompass the analytical coordinates necessary for the unambiguous detection and quantification of the target candidates [ 14].
These reference maps constitute highly specific protein assays including all essential coordinates of informative peptides (such as the mass, charge state distribution and chromatographic retention time of the precursor ion as well as the mass, charge state distribution and relative intensities of the fragment ion signals).
