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Stable, crypt-restricted immunopositivity for metallothionein (MT) is a more recently described stem cell mutation marker for mouse colon that can be assayed in paraffin-fixed tissue sections and has been validated against the G6PD assay (Cook et al, 2000).
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Cook, cook, cook, cook, cook.
Suitability of PCR assay was confirmed in raw (n = 20), cooked (60, 80 and 100 °C), autoclaved (121 °C) and micro-oven processed pork.
These were included to examine the performance of the assay across a range of sample types, including various cooking methods (fried, baked, boiled, grilled, cooked in sauce) and serial DNA dilutions (50, 40, 30, 20, 10 ng/μl).
For cooking status, we had four categories: never cook at home, cook monthly, cook weekly and cook daily or more.
All species from 12 commercial raw fish and five species out of eight cooked bonito fillets were successfully identified by the multiplex PCR assay.
The specificity of the assay to Atlantic cod was measured by testing highly processed food samples including frozen, defrosted and cooked fish fillets as well as fish fingers, battered fish fillet and fish pie.
Make assay!
Cooks cook.
Cook National Park, New Zealand.
Assay principle.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com