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As assay control, spiked samples (0.5 EU/ml) of the unknowns were measured in duplicate.
The calcium ionophore ionomycin (1 μM) was added at the end of each time course as an internal assay control.
Sterile discs of 6 mm width were dipped into the extract, AgNP solution, and extract stabilized AgNP as well as the assay control antibiotics.
To qualify the assay method, validation has to be conducted and an assay control strategy has to be determined by demonstrating that the assay performs according to its intended use.
For MTT assay, control samples were designed as follows: control group (cell culture with DMEM medium); positive control (1.0% Triton X-100, Sigma-Aldrich, USA); and negative control (chips of sterile polypropylene Eppendorf, 1 mg mL−1, Eppendorf, Germany).
Isotype specific antibody processed soluble chromatin was maintained as a ChIP assay control.
Using a tethering assay, control mRNAs and mRNAs under miRNA repression were tracked in live samples.
As an internal assay control TNF-α mRNA expression was also determined.
In this assay, control and treated worms were washed off their parent plates using M9 buffer.
Using Illumina's Genome Studio, background adjusted β-values and assay control probe information was used for initial quality control.
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In the first assay, control-EGFP and DNRII-EGFP were plated in 96-well culture plates at 1,000 cells/well and treated with different concentrations of TGFβ3.
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