CGR developed the SNP assays, constructed the linkage map and assisted with crosses.
JS identified the candidate promoter, conceived of the study, developed the study design and analytical assays, constructed and ran the clinical database, performed statistical analyses together with RS and participated in manuscript preparation.
On the basis of the results from SGTP affinity profiling assay and SGTP/GTP competition assay, we constructed a heatmap to better visualize the SGTP binding affinity and selectivity for 34 quantified GTPases toward the unique P-loop binding motif.
In the T cell proliferation assay, constructs with a higher copy number of TCEs, such as (L2T2 4, were more antigenic, as long as tandem repeats were separated by spacers.
Luciferase assays: Luciferase assay constructs were created by ligating PCR products into pGL4-11 (Promega) upstream of the luciferase gene.
Four hours after plating, microglia were incubated with lentiviral γ-secretase assay constructs at MOI of 1-3 for 18 hours followed by replacement with fresh culture media.
Also, even in this relatively non-quantitative assay, constructs with two intact SBSs resulted in substantially higher growth than those with one (compare bww and cww with other constructs), suggestive of the simultaneous binding of multiple syntrophin molecules per dystrobrevin molecule, as expected [ 37].
Eight kinds of rules to predict Salmonella/microsome assay were constructed, and currently results of the assay on aliphatic and heterocyclic compounds can be predicted as accurately as +90%.
More recently, a better assay was constructed using laboratory-prepared phosphomolybdate [ 20] similar to the assay developed by Glenn and Crane [ 21].
A dose-response assay was constructed by transfecting a range of amounts, from 0 to 1000 ng, of the ZNF131 expression vector in the assay (pcDB was added as necessary to ensure equal amounts of DNA in each transfection).
