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The assay consists of a chip covered with DNA strands.
The assay consists of S1 nuclease protection, sandwich hybridization and signal detection.
The HRM assay consists of intercalating dye based real time quantitative PCR (qPCR) and melting curve analysis.
The assay consists of target-induced Ag+ dissociation from hairpin DNA and an isothermal circular strand-displacement polymerization (ICSDP) reaction.
The assay consists of a nested PCR targeting the cytochrome b (cytb) mtDNA locus with a blocking primer in the internal PCR.
In conclusion, the NASBA-ELISA assay consists of an alternative process for large-scale samples detection with semi-quantitative results and provides good clinical performance without resorting to expensive equipment.
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Our assay consisted of 51 sets of primers designed to amplify all exons of these genes.
We describe a screening ELISA and a confirmation assay consisting of immune-purification followed by separation with SDS-PAGE and revelation with Western double blotting.
In summary, the assay consisted of undiluted saliva sample/analyte (1 μL), biotinylated antibody (10 mM) and acceptor bead (40 μg/mL) mix and streptavidin donor beads (50 μg/mL).
A negative control included in each assay consisted of medium lacking HIV antigen.
The protein, the main component of the assay, consisted of a fragment of the hemagglutinin of the Influenza A/H1N1 virus (further details are given below).
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