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Luciferase reporter gene assay confirmed the binding of miR-3185 with Linc02527 (Fig. 4d).
In addition, transwell migration assay confirmed significantly reduced migration of cells overexpressing miR-215-5p miR-215-5p miR-215-5p
Ex vivo luciferase activity assay confirmed the presence of MSC-TF on days 8 and 14 after MI.
Luciferase reporter gene assay confirmed the binding of miR-3185 with ATG5 and ATG7 (Fig. 5f, g).
After recombinant expression in E. coli, the HNL activity assay confirmed cyanogenic activity of all three isoenzymes (Supplementary Fig. 5b).
The MTT assay confirmed that the scaffolds can support the attachment and proliferation of stem cells.
DPPH assay confirmed the retention of antioxidant potential of HSP in SNEDDS.
Cell cycle analysis and DNA ladder assay confirmed cell survival and genomic stability.
The MTT assay confirmed that the polymers and the formulations were non-cytotoxic.
The results obtained in EdU assay confirmed the reduction of cell proliferation induced by MLK4 knock-down in HCC1806 cells (Fig. 3b).
Tissue assay confirmed that cRGDyC-USPIO could specifically target aHSCs.
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