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Using the avirulent strain M. tuberculosis mc26230 [31], [31], bacteria could be readily detected by fluorescence following fluoromycobacteriophage infection in a similar assay configuration to that used for M. smegmatis (Figure 6).
Using the pre-clinical assay configuration in which rifampicin, streptomycin or kanamycin are added simultaneously with phage, a large number of samples can be analyzed in a short period of time (<1 minute for each sample by flow cytometry), and drug susceptibilities determined within 16 hours.
DD, GH, JI advised on assay configuration and targeting.
This assay configuration was used to determine the stability of the entire rod.
The assay configuration allows comprehensive examination of genetic and epigenetic elements governing Pmp22 transcription in its native chromatin context.
To validate the final HTS assay configuration, a screen of a small compound set (validation set) was run in triplicate.
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Initial reactivity data on 9 HBsAg assay configurations determined for 28 defined and quantitated HBsAg recombinant mutant antigens (29 ) have been confirmed by several groups.
These TSP assay configurations for allele-specific PCR support rapid, low-cost endpoint SNP genotyping, since they are not reliant on fluorescently-labeled primers or probes and they do not require individual assay optimisation to accurately assign a genotype.
Four basic assay configurations are possible, depending on whether the immunoreaction occurs on a solid phase (heterogeneous) or in solution (homogeneous) and whether the antibody analyte binding is monitored directly (non-competitive) or via displacement of a labeled antigen (competitive).
Although it is difficult to reliably compare sensitivities reported for different lanthanide chelates obtained for different analytes and assay configurations in different laboratories, the heterogeneous DELFIA, FIAgen, nanoparticle, and EALL-based assays are all capable of delivering sensitivity in the lower ng L−1 range.
However, while the rank order of activities of various Cas9-XNA combinations is maintained across the assays, the absolute activity of Cas9 was highly dependent on assay type and configuration.
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