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A TaqMan assay conducted for the above novel miRNAs in S. portulacastrum, which was collected from the natural habitat of S. maritima, showed amplification of all of the miRNAs that were expressed in S. maritima, including sma-miR2 and sma-miR5, which were not expressed in the rice cultivars (Additional files 7 and 9A).
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Assays conducted for longer time periods revealed that these Lys mutants do retain <0.5% of the wild-type activity (results not shown).
Additional mutants of the N-terminal tip of α-helix 3 of TFEα and their effect on dimerization with TFEβ Ni-affinity co-purification assays conducted for TFEβ-His co-expressed with TFEα (WT), TFEα K46E, TFEα D49T, or TFEα R51E/K52E.
For the external pH shift assays, the conditions were the same as the H2 production assays except that the assay was conducted for 12 minutes, and KOH or HCl was added 6 minutes after the start of the assay to change the external pH from 5.5 to 6.5 or from 6.5 to 5.5.
In the text the authors noted six MCF-7 assays, but it is unclear whether a single assay was conducted for each of the six stressor and extraction combinations (microwave, UV, autoclave, saline, and ethanol) or whether the whole series was completed six times.
The assay was conducted for 30 min.
The assay was conducted for 3 min at 55°C, and absorbance was monitored at 475 nm.
Also, MTT cytotoxicity assay was conducted for the scaffolds and no significant cytotoxicity was observed.
A modified chrome azurol S (CAS) agar plate assay was conducted for siderophore production as described in Milagres et al. [82] using either 1.0 mM (final concentration) Cu II) (as CuSO4) or Fe(II) (as FeCl3·6H2O).
The assay was conducted for 500 seconds for each mosquito.
The assay was conducted for 24h in a closed humid chamber with 80% humidity.
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