Undifferentiated Thp-1 cells had previously been shown to be useful when measuring erythrophagocytosis [27], but no high-throughput assay concerning Fc-mediated phagocytosis that could actually be used consistently on large sample sets existed.
Encouraged by the reviewers' suggestions we made significant improvements to the arousal assay concerning both the mechanical aspects of stimulus delivery and the analysis of collected data.
Furthermore, this result is anticipated to stress the importance of further assays concerning the modifying of universal design principles.
These original biocomposites were fully characterized and in vivo biological assays concerning the AD treatment were performed using a mouse model induced by 2,4-dinitrochlorobenzene (DNCB).
For whole cell biotransformation assays concerning expression optimization of the hydantoinase, the harvested cells were thawed on ice, washed twice with catalysis buffer and resuspended in the same buffer in a ratio of 10 mL buffer for 100 mL of harvested culture.
The above assays concerned introduction of proteins that originate in the rhoptry bulbs and are injected into the host cell.
This is also true for the analytical assays concerning sensitivity and specificity versus availability, cost, and technician time required [ 7].
In order to functionally characterise the consequence of the R234Q mutation identified, we performed two different assays concerning two processes, in which PTEN has been shown to play a key role, namely cell proliferation and apoptosis.
The use of a more complex yet functional virus neutralization assay minimizes concerns about cross-reactivity with flaviviruses of other serocomplexes (e.g., dengue virus) that occasionally circulate in the US population.
As PCR assay was concerned, the amplification was carried out in 50 μl reactant, using the outer primers F3 and B3.
