Sentence examples for assay completed from inspiring English sources

In this regard, a model DNA hybridization assay whereby a fluorophore-labeled target ssDNA specific to human immunodeficiency, Hepatitis C (Hep C), is probed by an anchor probe immobilized on thin gold films, is driven to completion within 1 min with microwave heating, as compared to an identical assay completed in ≈ 4 h at room temperature.

Following the room temperature incubation, ELISA wells were washed followed by the addition of goat anti-rabbit Ig-alkaline phosphatase (Southern Biotech, Birmingham, AL) and the assay completed as per our normal ELISA protocol.

Gp120-coated cells incubated with A32 or 17b Fab fragments were washed once prior to addition of the competitor antibody and the assay completed as described above.

A stably transfected human neuroblastoma line that expresses pathogenic forms of Aβ (MC65) provides a rapid in vitro assay for the neurotoxic effects of intracellular Aβ accumulation (a 'high-throughput' assay; completed in 24 72 hours).

Cells were treated with 5 nM TFIIS siRNA #2, or 5 nM S-siRNA in 6-well plates, incubated at 72°C for 72 hours, and the assay completed as recommended by the manufacturer.

An ERG gene break-apart assay, completed as previously described (Attard et al, 2008), demonstrated that an additional 155 cancers (36%) in this series contained ERG gene rearrangements, including one patient who had both ERG and ETV1 rearrangements in distinct foci of cancer in the same prostate, as reported previously (Attard et al, 2008; Clark et al, 2008).

These data together substantiate that methylation of lysine 37 does not appear to play a major role in transcription, as mutation of this histone residue results in no overt phenotype in all transcription-based assays completed to date.

Now with the misincorporation assays completed with the three other polymerases, we were able to compare their enzyme fidelity by comparing the extent of the primer extension beyond the first stop site (see "*" in Fig. 2).

Among assays completed with the new limit, C-peptide was measurable in 88% of the basal samples, 87 95% of stimulated MMTT samples, and 92 94% of stimulated GST samples.

In this study, seven patients with SFG rickettsioses may have been infected by R. helvetica (five patients) or R. conorii Indian strain (two patients), according to IF assays completed for some cases by Western blot and cross-adsorption studies.

Following initial time course experiments (binding assays completed 0 – 72 h post-treatment; data not shown) cell-surface expression of CXCR4 and CD26 was determined at 48 h for subsequent experiments (Tables  1 and 2).