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In the Matrigel invasion assay, cluster 1 cells revealed to be more invasive than cluster 2 and 3 cells (+37%; P < 0.01).
The other three clusters include at least half of the pharmaceuticals, most likely in the GPCR assay cluster from Figure 2. Cluster iv involves 149 chemicals active in 2 to 48 assays, enriched for CYPs, transporters, and nuclear receptor (subfamily 1).
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[145] Polystyrene NPs modified and not modified with carboxylic acids 20 and 40 nm 0.3 6.6 nM; 4 16 h Caco-2 L/D cell assay; clustering analysis; apoptosis assay Decreased cell viability.
Consider, for example, assay clusters for biogenic amine GPCRs and peptide GPCRs from the assay by assay similarity matrix.
The chemical similarities revealed known assay clusters (e.g., for bisphenols or heavy metal compounds) and previously unreported clusters (e.g., for pharmaceuticals).
Prominent assay clusters containing relatively homogeneous assay categories included GPCR (other), nuclear receptor (subfamily 3), kinase and phosphatase, and GPCR (aminergic).
In the current RT MLPA assay, additional cluster I genes and at least two genes for each of the clusters II-IV were added.
In this assay the cluster A PP2C HAB1 [41], which contains no KIM and is not able to inactivate MPK6 [26] was used as a control.
The respective OPA (oligo pooled assay) and cluster files can be found online with this paper (Additional file 7 and Additional file 8).
In each assay, anti-clusters of differentiation antigen 4 (CD4) mAb B4 was used as a positive control [19].
To test whether CX directly promotes angiogenic sprouting in vitro, we titrated CX in a cellular angiogenesis assay where clustered human umbilical vein endothelial cells (HUVECs) form sprouts in a three-dimensional collagen type I gel [32].
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