c Summary of clonogenic assay cell survival fractions for all treatment conditions in this study.
The biocompatibility of PU-IONs was investigated by MTT assay, cell attachment and cell staining.
The cell viability on electrospun fibers was conducted with water-soluble tetrazolium salt-1 assay (Cell Proliferation Reagent WST-1).
Cytotoxicity, inhibition efficiency, and corresponding mechanism on colon cancer cell line HT-29 were evaluated by MTT assay, cell proliferation curve, western blot, and flow cytometry.
MTT assay Cell viability was measured using 3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide (MTT; Sigma-Aldrich Company Ltd., Gillingham, Dorset, UK) assay.
For LDH release assay, cell culture medium was mixed with reaction buffer at a ratio of 1 1 and the absorbance at 340 nm was measured as above.
The total DNA levels were used to assay cell adhesion on day 1, and cell proliferation during the 7-day culture period.
[144] VO nanotubes Diameter, 15 100 nm 0.1 0.5 mg/ml; 4 24 h Caco-2 Neutral red assay Cell death caused by the nanotubes.
[140] Ag nanorods Length-to-diameter ratio, 4 1 0.4 nM; 4 days HT29 МТТ assay; cell count Cytotoxicity is related to surfactants on the nanorod surface.
The Hoechst 33258-stained nuclei were counted to assay cell proliferation, while alkaline phosphatase (ALPL) immunostaining was performed to investigate osteogenic differentiation.
Caspase-Glo assay, cell cycle analysis, measurements of mitochondrial membrane potential (MMP) and levels of reactive oxygen species (ROS) were used to evaluate apoptosis induction.
