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Tyrosine was taken as the standard for all the enzyme assay calculations.
A Dextranase concentration of 2.0 U/mL was used for the well-plate assay calculations, and a concentration of 5.0 U/mL was used for the microfluidic assay calculations.
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[160] CdSe/ZnS QDs modified with COOH or NH2 groups (COOH-QDs and NH2-QDs, respectively) 4 10 nm 2.5, 5, 7.5, 10, 15, and 20 nM; 1 3 cell cycles BEAS-2B HFF-1 TK6 Flow cytometry; transmission electron microscopy (TEM); ELISA; ROS assay; calculation of cell population doubling time; fluorescent microscopy The rate of QD uptake is considerably higher in BEAS-2B and TK6 cells.
Although individual sensitivity values could not be determined for all mutations assayed, calculations using unidirectional KRAS assays (reflective of the majority of OncoMap assays) yielded nearly identical sensitivity and specificity values (Figure S1).
Subsequent assay efficiency calculations were carried out in Light Cycler Relative Quantification Software (Roche Diagnostics).
For comparing significant distributions between different groups in the lifespan assays and stress resistance assays, statistical calculations were carried out using the log-rank test.
More details of clonogenic assay and calculation of survival fraction are shown in Additional file 1: Section S1.
Multiple measurements of PD-1 expression on Tetramer positive cells in a given assay enabled calculation of mean and standard deviation (SD).
Data on reproducibility of the assay and calculation of IgM and IgG levels are provided.
The assays and calculations were performed according to the manufacturers' protocols.
The assays and calculations were performed according to the manufacturer's instruction.
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