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One of the goals of the current work was to increase the throughput of the chemical screening assay by utilizing a commercial instrument to distribute a precise number of infected worms in each microtiter well.
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Receptor-induced intracellular release of calcium in response to agonist treatment was measured in a fluorometric assay by utilizing an imaging-based plate reader (FDSS6000, Hamamatsu, Japan) that makes simultaneous measurements of calcium levels in each well of a 384 plate.
E. coli libraries expressing mutant LovD were obtained, and the whole-cell activities of converting monacolin J to simvastatin were assayed by utilizing an agar-based diffusion screening assay.
Sensitivity of serum assays might be enhanced by utilizing a panel of biomarkers.
Some previous investigations have circumvented the need to control for group behavior by utilizing a shoaling assay to measure preference for social affiliation [1], [8] [15].
The mitochondrial DNA content was quantified by utilizing a fluorometric assay where an increase in SYBr Green (Invitrogen Molecular Probes) fluorescence because of higher DNA binding was quantified as a measure of the amount of DNA content.
We (i) challenged participants by utilizing a cognitive assay that manipulated the level of required cognitive control within the same task, and (ii) examined whether Met carriers would display a performance advantage only when the cognitive control requirement was low, and Val carriers would perform better when the demand for cognitive control was increased.
FLS cell motility was evaluated by utilizing a scrape motility assay.
MLPA assay has been used for LOH detection by utilizing a heterozygous LGR region as a marker for LOH, similarly as described before [ 1, 34, 35], however LOH was calculated by using a formula: exon dosage quotient in the tumor DNA/exon dosage quotient in the germline DNA, that was applied on the exons affected in the germline.
Therefore, we analysed the ability of PKD-null DT40 B cells that lack expression of all PKD isoforms to activate Rap1 by utilizing a Rap1 pull-down assay.
The pull down assay was performed with the purified GST-FtsZ or GST proteins by utilizing a modified method (Dziedzic et al., 2010).
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