Sentence examples for assay by using the from inspiring English sources
Cells treated with TGF-β (5 ng/mL) for up to 48 h were harvested and processed for apoptotic assay by using the Annexin V-PI double staining as described in 'METHODS AND MANDRIAND'.
The release of phosphate from the substrate was measured in a colorimetric assay by using the Biomol Green Reagent in accordance with the instructions of the manufacturer.
Embryos were subjected to TUNEL assay by using the ApopTag Red in situ Apoptosis Detection Kit (Chemicon, Temecula, CA) according to the manufacture's instruction.
Isolates were tested in the NI assay by using the NA-Fluor kit (6).
The epitope specificities were detected by enzyme-linked immunosorbent assay by using the recombinant fragments as solid-phase ligands.
Apoptosis was measured with TUNEL assay by using the TUNEL-based ApopTag Peroxidase In Situ Apoptosis Detection Kit from Millipore (Temecula, CA, USA), as per manufacturer's instructions.
The optimized reaction conditions (such as pH and temperature), thermal stability, and reusability of the immobilized enzyme on PE films were assayed by using the enzyme-catalyzed reaction.
The bindings of the probes were additionally investigated by microtiter plate assays by using the most effective ligands determined by CoMPP, with the addition of related polymers (Fig. 4).
At 24 h after transfection, reporter activity of samples was assayed by using the Dual Luciferase Assay System (Promega).
