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Fig. 2 Reaction of biofilm formation in microtiter plate assay by a clinical isolate 4 of Staphylococcus epidermidis.
The LSCC-RP9 cells (avian leukosis virus-transformed B-lymphoblastoid cell line from chickens) were used as target cells of PBMC in the peripheral blood for NK cytotoxic activity assay by a flow cytometry using the DiOC/18 membrane dye (Molecular Probes, Eugene, OR) to stain alive LSCC-RP9 cells and PI nuclear dye to stain the dead cells (Kushima et al. 2003).
Three µg nuclear extract was used for SRE transcription assay by a kit from Marligen Bioscience (catalog # 11944-096 and 11906-100) according to manufacturer's instructions.
Whole-blood samples were also cryopreserved at enrolment, for HIV DNA assay by a centralized laboratory using the ANRS real-time PCR assay [16].
Positive responses were determined in the ICS assay by a one-sided Fisher's exact test applied to each response to a peptide pool vs. the response to the negative control with a discrete Bonferroni adjustment for the multiple comparisons.
Experiments were carried out 48 hours post-transfection the cells were either counted for the proliferation assay by a haemocytometer or lysed with lysis buffer for the immunoblot analysis.
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BNP was measured using an immunoradiometric assay by an external laboratory (SRL Inc., Tokyo, Japan).
Simultaneous exposure to tamoxifen or perhexiline decreased resistance to adriamycin in a clonogenic assay by an undefined mechanism [ 30].
The migration ability of RH30 cells stably overexpressing TBX3 was assayed by a scratch wound assay and we found that TBX3 also inhibited cell migration as assayed by wound recovery (Fig. 3d).
We performed transwell assays by a two-chamber migration system, in which, THP-1-derived macrophages and Cal-27 were separated by an 8 μm pore membrane.
Umbilical cord plasma interleukin-6 concentrations were assayed by a sensitive and specific immunoassay.
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