The compounds were highly potent in combination with high efficacy in a rat pituitary cell assay, being characterized with EC50 values down to 0.8 nM.
The two options also support different mixing approaches being considered for a highly sensitive companion TB-screening assay being developed at Northwestern University (XtracTB assay).
These molecules exhibited an extraordinary high in vitro potency in the chick heart invasion assay, being active up to 10 nmol L−1, a concentration level a 100-fold lower than the lowest effective doses that have been reported for natural analogues.
The values returned from analysis of feces, water and soil samples are in general agreement between the assays (Pearson correlation r = 0.9828, P < 0.001), with the ND5 assay being consistently more sensitive (Tables 2, 3).
After the 24-h exposure, cells were incubated for 3 h with 500 μg/ml MTT reagent or for 2 h with 100 μg/ml neutral red dye, depending on the assay being performed.
As operated here, LAMP is an exponential rate assay being assessed with an endpoint measurement.
