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DNA extracted from cervical cells of women aged ≤ 24 years, was additionally tested for the 13 HR types plus HPV6 and 11 using an alternative PCR-based assay based upon multiplex E7 gene PCR and DNA microarray [ 9] at IARC.
This study investigated a dual-fluorescence assay based upon the LIVE/DEAD® BacLight™ Bacterial Viability Kit to quantify the percent viability of filamentous cyanobacteria using a microplate reader in a high throughput 96-well plate format.
A recently developed novel and efficient screening assay based upon expression of a dicistronic mRNA (RHO IRES SEAP) containing both RHO and reporter (SEAP) cDNAs was used to compare the hhRz 266 lead candidate to another agent (Rz525/hhRz485) already known to partially rescue retinal degeneration in a rodent model.
For culturing cells as spheres we have used the mammosphere assay based upon culturing neural stem cells in serum-free medium containing recombinant human epidermal growth factor (rhEGF) and basic fibroblast growth factor (rhbFGF) [11], [19].
We therefore developed a detection assay based upon PNA chemistry.
Third instar nociception assay based upon nocifensive characteristic escape behaviours to heat (17, 28).
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However, the introduction of assays based upon various nucleic acid testing (NAT) technologies, each utilizing a different set of standards, creates the potential for misinterpretation of patient results.
We have designed and evaluated four assays based upon PCR amplification of short interspersed elements (SINEs) for species-specific detection and quantitation of bovine, porcine, chicken, and ruminant DNA.
These results suggest that functional assays based upon endogenous miRNA expression may be another means to assess properties of hiPSC.
Molecular diagnostic assays based upon reverse transcriptase-polymerase chain reaction (RT-PCR) have short assay cycle time, and high analytical sensitivity and specificity.
Xylanase activity was also assayed based upon the color reaction between the degradation products (xylose) and DNS.
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