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In contrast, the percentage viability obtained for MTT assay at the same concentration of LPG (50 µg/mL) treatment was 17.2 ± 1.47%, 35.26 ± 2.9% and 79.01 ± 1.59% for HepG2, HeLa and CC1 cells respectively.
We subcultured cells for migration assay at the same time.
Specimens were tested using the same HPV assay at the same testing laboratory ensuring consistency of results.
When we performed matrigel invasion assay at the same condition, KLF17 overexpression inhibits cell invasion significantly in both the cells (P<0.005).
Similar(56)
Parallel cohorts were assayed at the same time under the same conditions and the experimenter was blinded to the genotypes or experimental conditions.
Cell supernatants were collected for virus titration and soluble factor assays at the same times that RNA was harvested; the supernatant was frozen at −80°C until subsequent analysis.
Standards (0.5, 1, 2, and 10 µM) of glutathione and a sample blank lacking glutathione were also assayed at the same time.
Unmanipulated HT1080 cells were assayed at the same time.
This assay allowed us to run many assays at the same time.
Concentrations of serum C-reactive protein (CRP) and plasma fibrinogen were assayed at the same time.
For each experiment, PBMCs from all individuals were thawed and assayed at the same time.
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