Comparing these values to significant downregulation of 69%to88%8% of all identified kinases by our assay argues for a high specificity of kinase level reduction after Hsp90 inhibition.
Together, both in vivo and in vitro assays argue that instead of causing defects directly on migration, Nf1 inactivation promotes premature neuronal differentiation of a subset of GCPs, leading to abnormal accumulation of differentiated neurons in the ML and EGL.
The fact that 8 of the 16 posterior Hox genes from all posterior paralogs groups except Hox12 show an effect in the ingression, elongation and Wnt signaling assays argue for an extreme redundancy of the system that could explain the intraspecific robustness of the vertebral formula.
That 8 of the 16 posterior Hox genes from all posterior paralog groups except Hox12 show an effect in the ingression, elongation, and Wnt signaling assays argue for an extreme redundancy of the system that could further explain the intraspecific robustness of the vertebral formula.
We found that neither compound inhibited the activity of other HECT-domain ubiquitin ligases in these assays, arguing that they are specific inhibitors of HUWE1 (Fig 3C).
Moreover, APMAP1 purified from E. coli or CHO cells did not interfere with the processing of APP-CTFβ in our cell-free γ-secretase activity assays, arguing against a direct effect of APMAP on the activity of the mature γ-secretase (Supplementary Material, Figs S7 and S8).
We also introduced a K45E substitution within the α domain of scIHF3 and based on electrophoretic mobility shift assays (EMSAs), argue that it significantly changes the DNA trajectory within the protein DNA complex.
However, no annexinV-positive/propidium iodide-negative cells, indicative of apoptosis, were observed under any assay conditions, arguing against induction of apoptosis.
In additional, the MEP score was found to be predictive across 3 separate assay platforms, arguing for its broad applicability and against experimental artifact.
Moreover, the results of ChIP assay also strongly argue that Jdp2 exists in vivo at different ARE sequences and its recruitment can be enhanced by TPA.
