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J.K. and H.J.H. designed the enzyme assay and kinetics studies.
Total cellular protein levels were measured by the Bradford protein assay and used for normalization.
After compound treatment, luciferase activity assay and the activity fold change were determined as described above.
Phospho-CrkL (pCrkL) assay and cell cycle analysis were also conducted.
Cell proliferation and apoptosis were detected using CCK-8 assay and flow cytometry, respectively.
The enzymes used with each assay and each cross are detailed in Supplementary Table 1.
Wound-healing assay and transwell assay were performed following protocols as we described previously.
performed the NQO1 assay and KEAP1 wild-type and Cys151Ser mutant experiments.
Stocks were prepared, quantified by plaque assay and sequence verified by Sanger sequencing.
High-density multiplex detection of nucleic acid sequences: oligonucleotide ligation assay and sequence-coded separation.
Frozen sections of 50 μm were used for lectin perfusion assay and PODXL staining.
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