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Total lung collagen measurements were made as described previously [ 16], using the Sircol collagen dye-binding assay (Accurate, Westbury, NY, USA).
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In both assays accurate fold changes are difficult to calculate, if the baseline expression level is close to background noise or the detection limit.
The simplex PCR assay enabled accurate quantification of the different alleles in the mix.
The matrix-assisted laser desorption ionization time-of-flight mass spectrometry-based restriction fragment mass polymorphism (RFMP) assay provides accurate, broad-spectrum, high-throughput genotyping of HPV.
The methylation status of LINE-1 was evaluated by AQAMA assay for accurate assessment of CpG island methylation levels [31].
The new assay provides accurate diagnostic results in less time than currently employed conventional PCR-assays.
In this case, successive experiments can result in compounded errors and makes the assay less accurate.
In 1980, the first latex agglutination assay with accurate identification of S. aureus was introduced by Essers and Radebold [ 1].
These results demonstrate that the newly established assay was accurate and reproducible across a range of LINE-1 methylation levels.
The E-cadherin assay was accurate enough to quantitate the concentration of soluble E-cadherin in the cell culture supernatants.
Thus, this assay is accurate, reproducible and easy to handle, and nicely complements qualitative microscopy-based methods.
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