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To explore structure-activity relationship of DC07090, 15 new derivatives were designed, synthesized and evaluated in vitro enzyme assay accordingly.
The immunohistochemistry evaluation of gastric tissues showed the protein regulation in situ which was confirmed by western blot assay accordingly.
In the revision, we have elaborated the nucleotide exchange assay method in the Materials and methods section (Nucleotide exchange assay) accordingly.
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DEX binding assays accordingly demonstrated a 50% decrease in the number of fibroblast hormone-binding sites as compared to controls (Fig. 4D).
Thus, the failure of LI08 to appreciate the full scope of variation at Gp-9 and design assays accordingly could explain their inability to detect relevant GP-9 variation using PAGE in three of four ostensibly polygyne colonies.
The early passage (< 5 passages) fibroblast strains were plated at a density of 2.5 × 105 cells in 35 mm plates and grown for assays accordingly.
The optimized compounds were synthesized and subjected to EGFR binding assay and accordingly some more potent inhibitors were obtained.
For example, the previous presence of anti-dsDNA antibodies (by any assay) and accordingly a positive ANA test will qualify a patient with a UV-sensitive rash for SLE classification and diagnosis, whereas clinical manifestations do not coincide with anti-dsDNA antibody detection.
Separate sets of NAG cells were employed for the Aβ40 and Aβ42 assays, and accordingly, there are two separate assays for PBA effects upon AICD production.
The same −4/−1 pre-melted T6 promoter template was used in EMSA and permanganate assays, which accordingly result in a strong signal at −1.
It was found that the popular Cheng Prusoff equation is invalid for the calculation of the competitive inhibition constants (Ki values) for inhibitors in the FP-based competitive binding assay conditions, and accordingly, a new mathematical equation was developed, validated, and used to compute the Ki values.
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