Sentence examples for assay accessibility from inspiring English sources

Key advantages of using DAM to probe eukaryotic chromatin structure are (i) the ability to assay accessibility in a living cell, (ii) the ability to assay designated sets of cells or tissues in complex biological samples using transgenes with regulated promoters, and (iii) the lack of any known background of DAM activity in eukaryotes.

Three-dimensional (3D) Stochastic Optical Reconstruction Microscopy (STORM) demonstrated that, after entering into the autophagosome, IL-1β colocalizes with LC3 on the autophagosomal membrane, which, together with an antibody accessibility assay and observations from biochemical assays, implies a topological distribution in the intermembrane space of the autophagosome.

Therefore, we tailored a focused restriction enzyme (BglII) accessibility assay [26] for the analysis of ARS1 on chromosome IV.

We verified this response to dynamin depletion using the fluorescent probe for the endogenous scavenger receptor, mBSA, in an accessibility assay (Figure 4C).

It is noteworthy that a similar DNAase accessibility assay has failed to detect variations in the structure of ARS1 between wt and orc2-1 cells [18], yet it is known that ARS1 activity is severely impaired in orc2-1 cells [31].

Although previous immunofluorescence studies indicated that BbCRASP-2 antibodies recognized native antigen on the surface of the intact spirochetes [17], our proteinase K accessibility assay indicated that BbCRASP-2 is not significantly exposed on the spirochete surface (Fig. 2B).

In a chromatin accessibility assay, we found the promoter region of PHD3 in PC-3 cells was resistant to cutting by DNase I when compared to the MCF7 promotermoter, whereas there was little change in GAPDH promoter accessibility between the two cell lines (Figure S2).

RJW helped develop and implement the chromatin accessibility assay for this study.

To address this question, we have used an entirely purified in vitro system and a restriction enzyme accessibility assay (REA).

Within an in vitro accessibility assay the TC-NER-specific CSB disarranged regularly spaced nucleosomes in an ATP-dependent manner [ 102].

A chemical assay of the accessibility of the acyl thioester to solvent revealed a positive correlation between chain length and rate of hydrolysis.