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Through a high throughput screening assay, a set of compounds were found able to inhibit the enzymatic activity of cathepsin B. The binding structures of these active compounds were modeled through docking simulation.
In this assay, a set of forward and reverse primers were designed targeting the serotype conserved region at the NS5 gene, at the same time flanking a variable region for all four serotypes which were used to design the serotype-specific TaqMan probes.
For the single colony formation assay, a set of serial dilutions was performed, and the number of viable ES cell colonies was assessed with alkaline phosphatase (Millipore).
In order to assess the analytical sensitivity of the QuanTAS-PCR assay, a set of mutant/wild-type mixes were prepared by mixing Cq normalised HEL DNA (MUT) and HL-60 DNA (WT).
Excellent agreement with multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) results [ 13] was reached using in the PCR/ESI-MS assay a set of eight primer pairs that target the same genes as those for MLST.
To address this, the authors should include in this assay a set of negative controls, including a construct lacking luciferase, and a set of contexts with very low RTP scores, to establish a background readthrough rate.
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We assayed a set of anti-CD20 IgG1 variants that had identical Fab regions, but alterations in the Fc regions, in both the soluble receptor-based and cell-based FcγRIIIa binding assays.
We assayed a set of biochemical parameters to estimate the possible interference from either wild-type or mutant HPV-16 E7 with the cellular redox equilibrium.
Bataillon et al. 30 later assayed a set of favorable mutants in a large set of very simple environments (95 different carbon sources).
We next assayed a set of peptides that were designed to determine whether optimization of the residue adjacent to the N-terminal Arg (at the −2 position to the acetylated lysine) would lead to improved activity and specificity.
In his plenary lecture, John Butler noted that the mini-satellite marker systems have stood the test of time and the high levels of polymorphisms continue to contribute to the DNA profile database that assays a set of 15 simple tandem repeats (STRs).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com