Even though the same RNA samples were used in both microarray and qPCR-array assays, a low correlation (r = -0.443) was observed with miRNA expression indicating the variation associated with the two platforms.
The sensitivity of our neutralizing assay is comparable to that of ELISA (9 ), but the possibility that our assay had a low sensitivity remains because the neutralizing antibody test is based on the reading of a complete inhibition of cytopathic effect.
The brain-specific proteins full-length amyloid β1-42 and 1-40 (A β1-42 And A β1-40) and truncated A β-42 and -40 (A βN-42 and A βN-40) were determined in plasma by using a simultaneous Luminex assay (INNO-BIA plasma A β forms; Innogenetics, Ghent, Belgium), which has been shown to be a reliable assay with a low variability [ 27].
Cytotoxicity assay showed a low cytotoxicity of PAE-g-ODA-2 micelles as well as a higher inhibition against HepG2 tumor cells of DOX-loaded micelles than free DOX.
A fluorometric glutathione detection assay with a low detection limit (153 nM, at an S/N ratio of 3) can be designed via this method.
The Alamar Blue assay provided a low initial cell attachment (40%) and cell numbers were similar on both the uncoated and coated Ti scaffolds after 3 days.
The sensitivity of the ProC® Global assay for a low protein S (PS) level (n=56) was only 66%, and was even weaker in the case of hereditary PS deficiency (46.6%, n=15).
It is a measure of the imprecision of an assay at a low analyte concentration and involves variation due to measurement imprecision and not to biological variations.
To analyse the effects of VEGF-A and COX-2 downregulation mediated by HuR knockdown in TECs, we performed a cell proliferation assay under a low serum condition.
Serological assay has a low cost and does not rely on PCR amplification, but it is still subjected to the effects of specific base mutation.
